| Congresso Brasileiro de Microbiologia 2023 | Resumo: 193-1 | ||||
Resumo:Abstract
Candida auris is a yeast first isolated in Japan about a decade ago. Since then, it has been isolated on all continents and has become a global threat due to its high capacity to develop resistance to the main classes of antifungals (azoles, polyenes and echinocandins), compromising the treatment of infections. It presents even less susceptibility to disinfectants commonly used in hospital environments, facilitating its permanence in these places. In 2020, the first outbreak of C. auris in Brazil was reported, in a hospital in Salvador, Bahia. Since then, other outbreaks have been reported, one more in Salvador, in 2021, and in Recife, in 2022. In 2023, was reported the first case in São Paulo state. In this scenario, natural products have been widely studied in order to find molecules with antimicrobial potential. Over the past forty years, more than half of the organic compounds approved for human use have been derived from natural products. Among these compounds, few with antifungal action were approved due to cytotoxicity to human cells due to the fact that fungi are eukaryotic organisms and phylogenetically close to animals. Eugenol and cinnamaldehyde, major compounds of essential oils of clove and cinnamon, respectively, have antimicrobial action against different species of Candida spp.. Our aim is to analyze the action of these compounds on C. auris isolates. Sensitivity tests were performed in 96-well microplates to verify the Minimum Inhibitory Concentration (MIC) of the compounds in 25 C. auris isolates from different geographic clades, one isolate from the first outbreak in Brazil, and an ATCC standard. After the incubation period of the plates, subcultures of the content of the wells in Petri dishes were performed to determine the Minimum Fungicide Concentration (MFC). Survival curves of 5 isolates from different clades, one isolate from the first outbreak in Brazil and an ATCC standard, were constructed using 1&frasl4 of the MIC of cinnamaldehyde and eugenol and 1&frasl4 of the MIC of itraconazole. MIC values ranged between 31.25 and 250 &mug&fraslmL for eugenol and between 31.25 and 62.5 &mug&fraslmL for cinnamaldehyde. Eugenol and cinnamaldehyde showed synergistic fungicidal interactions with itraconazole.
Development Agency: Fundação de Amparo à Pesquisa do Estado de São Paulo, processo Nº 2022/07036-0 Palavras-chave: Antifungal resistance, Candida auris, Cinnamaldehyde, Eugenol Agência de fomento:Fundação de Amparo à Pesquisa do Estado de São Paulo | |||||